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Prothrombin fragment 1+2

Item Value
Available Stat? No
Test code PROF
Performed by Quest
Sendout? Yes
Method Enzyme Immunoassay
Collection Instructions 1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.

2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.

3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.

4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.
Container type Blue top filled to full extent of vacuum
Amount to Collect 2.7 mL blood
Sample type Citrated plasma
Preferred volume 1 mL plasma
Min. Volume 0.5 mL plasma
UCSF Rejection Criteria Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected
Processing notes Deliver sample to Hematology ASAP for processing.

Freeze plasma in 1 mL aliquots at -20C. Ship on dry ice Monday-Friday to Quest, test code 37674X.

For B &T patients ship on dry ice to LabCorp.test code #500016.
Units pmol/L
Normal range 41-372 pmol/L
Turn around times 2-10 days
Additional information When prothrombin is converted to thrombin the prothrombin fragment 1+2 is released from prothrombin. The test is useful to assess ongoing coagulation activation as may occur in thrombosis or DIC.
CPT coding 83520-90
LOINC code 27824-2
Last Updated 6/12/2014 5:47:27 PM
Entry Number 1094
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