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Alpha-fetoprotein, amniotic fluid (with reflex to Acetylcholinesterase and Hgb F)

Item Value
Test Update Information Effective October 4, 2010, the Amniotic fluid AFP test is being performed in Chemistry China Basin on the Siemens Centaur chemiluminescent immunoassay instrument
Approval req'd? No
Available Stat? No
Test code AFPX
Test group Alpha-fetoprotein
Performed by China Basin Chemistry (Quest for reflex Acetylcholinesterase and Hgb F)
Sendout?
Amniotic fluid AFP No
Acetylcholinesterase & Hgb F Yes
In House Availability Test performed Monday, Wednesday and Fridays. Results reported by 1600.
Method
AFP Chemiluminescent Immunoassay (Siemens Centaur)
Acetylcholinesterase Gel electrophorsis,
Hemoglobin F Radial immunodiffusion,
Collection Instructions Avoid contaminating the fluid with blood. Gestational age (15 to 24 weeks) must be provided for interpretation of results. Indicate gestational age, collection date, and clinical indications, if available, on the Cytogenetics test requisition form.
Container type Clean container
Amount to Collect 3 mL amniotic fluid
Sample type Amniotic fluid
Preferred volume 3 mL amniotic fluid
Min. Volume 1.2 mL amniotic fluid
UCSF Rejection Criteria Bloody samples.
Processing notes Refrigerate sample and transport to China Basin Cytogenetics

If reflex testing is required, acetylcholinesterase and hemoglobin F (Quest order code 8284N) will be entered and billed for services performed when tests are completed.
Units µg/mL (mcg/mL)
Normal range
MOM < 2.00
Acetylcholinesterase Neg
Fetal Hemoglobin Neg
Synonyms AFP; Alpha-fetoglobulin
Stability Room temperature 2 weeks, refrigerated 3 weeks, frozen at -20C 1 year.
Additional information Based on AFP result the sample will also be tested for acetylcholesterase and fetal hemoglobin. If reflex testing is required, the required tests will be entered and billed as performed.

Amniotic fluid samples collected before 15 weeks gestation will be sent to Quest Diagnostics for testing due to no in-house established MoM for these early gestational ages. Quest order code 232Z.
CPT coding 82106
LOINC code 1832-5
Last Updated 3/24/2013 7:06:13 PM
Entry Number 1092
Lab Procedure Link Click here for Procedure
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