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Lab Manual for Moffitt-Long and Mount Zion

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BCR/ABL translocation FISH

Item Value
Approval req'd? No
Available Stat? No
Test code TR922
Test group BCRABL
Performed by Medical Genomics - Cytogenetics
Method Fluorescent in-situ hybridization
Collection Instructions Click here for Neoplastic Cytogenetics Requisition
Container type Blood & bone marrow aspirate: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844. Dark Green top also acceptable

Bone marrow core: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
Amount to Collect
Bone marrow 2 mL
Blood 2 mL
Bone core 2 cm
Sample type Heparinized whole blood, bone marrow, bone core
Preferred volume
Bone marrow 2 mL
Blood 2 mL
Bone core 2 cm
Min. Volume
Bone marrow 1 mL
Blood 1 mL
Bone core 1cm
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.
Processing notes Maintain sample at room temperature. Transport to CB Cytogenetics within 24 hours
Normal range Not detected
Synonyms PCR; CML; Chronic mylogenous leukemia; Philadelphia chromosome; PH1 chromosome; Breakpoint cluster region; Tyrosine kinase; Translocation 9:22; t(9:22); Ph' chromosome; Philadelphia chromosome; ALL; PH1 chromosome; Cytogenetic analysis; Karyotype; Karyotyping
Stability 48 hours
Turn around times Set up Monday-Friday only. Results available 48 hours after set-up.
CPT coding 88271ZS, 88275ZS
LOINC code 51867-0
LDT or Mod FDA? Yes
Last Updated 1/22/2014 8:57:16 AM
Entry Number 1088

If you have additional questions regarding this test, please call: 415-353-1667

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