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Lab Manual for Moffitt-Long and Mount Zion

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Barbiturates Screen, Urine

Item Value
Test Update Information Effective 01/14/2013, the platform used for the barbiturates immunoassay screen has changed from the Abbot Axsym to the Beckman UniCel DxC 800.
Approval req'd? No
Available Stat? Yes
Test code BARB
Test group Barbiturate
Performed by Parnassus Chemistry
In House Availability Test available 24 hours per day 7 days per week
Method Competitive enzyme immunoassay method (Beckman UniCel DxC800) using G6PDH labeling.
Container type Urine cup
Amount to Collect See preferred volume
Sample type Random urine
Preferred volume 1 mL urine
Min. Volume 0.5 mL urine
Units µg/L
Normal range Negative

Note: a negative result indicates that this class of drugs is not present, or they are present at a concentration below the cutoff concentration of 200 µg/L
Synonyms Butisol; Alurate; Amobarbital; Amytal; Apobarbital; Butabarbital; Butalbital; Fiorinal; Gemonil; Metharbital; Secobarbital; Seconal; Tuinal
Stability Refrigerated 1 week, frozen at -20C 2 weeks
Turn around times Stat 2 hours, routine 4 hours
Additional information A concentration of < 200 µg/L is considered negative by this test. A positive result is ≥ 200 µg/L and indicates the presence of this class of drugs. This immunoassay is only a screening test and is not definitive. Results cannot be used for medico-legal purposes.

This assay detects a number of barbiturates at varying concentrations.

Click here for List of Cross Reactive Substances

False positive results may occur due to other cross reacting substances, however, positive results are NOT routinely confirmed by a second method. If confirmation of the test result is required, it is the responsibility of the physician to separately order a specific confirmatory test for the drug identified. Samples are held for 7 days. Contact laboratory at x 31667 to request confirmatory testing. Confirmatory test code BARC. False negative results are also possible.

Barbiturates can be detected in urine from 1 up to 14 days after use (especially for phenobarbital as it is long-acting).
LOINC code 20664-9
Last Updated 3/24/2013 7:38:04 PM
Entry Number 107
Lab Procedure Link Click here for Procedure

If you have additional questions regarding this test, please call: 415-353-1667

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