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QuantiFERON Gold TB

Item Value
Approval req'd? Yes, inpatients, except liver and kidney transplant service, require approval by ID service
Available Stat? No
Test code QNTFP
Performed by Quest
Sendout? Yes
Method ELISA
Collection Instructions Note it is mandatory the the tubes be filled correctly as underfilled or overfilled tubes will be rejected by Quest.

INPATIENT:
COLLECT SAMPLE 4 AM - 2 PM ON MONDAY-THURSDAY ONLY. DO NOT COLLECT SAMPLE ON UCSF OBSERVED HOLIDAYS.

OUTPATIENTS:
COLLECT BETWEEN 7 AM – 2 PM ON MONDAY – THURSDAY BY UCSF LABORATORY STAFF AT OUR OUTPATIENT FACILITIES


1. For Parnassus and Mission Bay inpatient only, pick up collection
kits from Laboratory Central Processing. Outpatient clinics will have
their own collection kits.
2. Collect 1 ml of blood by venipuncture directly into each of the three
tubes (Nil, TB Antigen, Mitogen). Fill each tube up to the black
mark. Under or overfilling of the tubes may lead to erroneous
results.
3. Mix the tubes by shaking firmly and vertically for 10 times and label tubes appropriately.
4. These are time sensitive samples, please deliver the tubes to the
Laboratory Central Processing immediately.
Container type Requires special collection kit available from central laboratory containing:

QFT-Nil Control tube Gray cap, white ring
QFT-TB Antigen tube Red cap, white ring
QFT-Mitogen Control tube Purple cap, white ring


Note it is mandatory the the tubes be filled correctly as underfilled or overfilled tubes will be rejected by Quest.
Amount to Collect 3 mL blood, place 1 mL of blood into each tube in the collection kit
Sample type Whole blood
Preferred volume 3 mL blood, place 1 mL of blood into each tube in the collection kit
Min. Volume 3 mL blood, place 1 mL of blood into each tube in the collection kit
UCSF Rejection Criteria Frozen samples. Improperly collected samples.
Processing notes CENTRAL PROCESSING STAFF:
Accession the samples immediately upon receipt and send to Immunology at China Basin in room temperature. Samples received after 2 PM please verify with China Basin processor before rejecting
Ref Lab Rejection Criteria Overfilled or underfilled tubes received.
Normal range Not detected
Synonyms Tuberculosis; TB; TB skin test; Interferon gamma; IFN gamma
Stability Samples need to be incubated at China Basin as soon as possible after collection (within 16 hours of collection).
Turn around times 5-7 days
Additional information The QuantiFERON test detects the release of IFN-gamma released by whole blood lymphocytes after incubation with tuberculin purified protein derivative and phytohemagglutinin.

The test is intended for screening asymptomatic individuals who may be at risk for latent tuberculosis (recent immigrants, prisoners and prison/jail employees) or TB exposure (military, hospital personnel). In these settings it is equivalent but no better than skin testing.

QuanitFERON testing may be more specific than skin testing when screening individuals with a history of prior BCG vaccination.

It is NOT recommended for use in patients with symptoms of active TB (known to cause decreased IFN-G responses), contacts of patients with active TB, immunosuppressed patients, patients with diabetes, silicosis, chronic renal failure, leukemia, lymphoma, head/neck cancer, lung cancer, s/p gastrectomy or jejunoileal bypass. In these situations skin testing is recommended.
CPT coding 86480-90
Last Updated 9/26/2016 5:21:09 PM
Entry Number 1061
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