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Urine Culture and Gram stain (restricted to surgical collection)

Item Value
Approval req'd? No
Available Stat? No (gram stain may be requested stat)
Utilization Guidelines Urine collected surgically and Gram stain may be used to modify treatment (intra-op / post-op administration of antibiotics).

Urine Culture and Gram Stain is restricted to surgical collection, other patients should have Urinalysis (with urine culture if UA shows pyuria) ordered. See separate lab manual entry for Urine Culture.
Test code P059S
Test group Bacterial Culture
Performed by Microbiology
In House Availability Set up daily, day and evening shifts
Method Aerobic culture
Collection Instructions Submit voided urine in a cup with a screw-on lid. Use a Red top vacutainer to collect the specimen from a bladder catheter. Specify whether a Gram stain is desired. The physician can, by checking the appropriate box on the requisition, select a screening culture for significant numbers of a presumptive pathogen only and decline organism identification and susceptibility testing.
Container type Urine cup
Amount to Collect See preferred volume
Sample type Random urine
Preferred volume 10 mL urine
UCSF Rejection Criteria More than one midstream or indwelling cath urine in 48 hours
Normal range See Additional Information
Stability Refrigerated 24 hours
Reflex? If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed.

Full ID and/or susceptibility testing may be omitted upon request when sample is submitted for testing.
Additional information Colony counts on urine cultures with growth are reported as follows:

1,000 CFU/mL
10,000 CFU/mL
100,000 CFU/mL
≥1,000,000 CFU/mL

Based on the use of a calibrated 10 µl loop used to inoculate plates, each resultant colony represents approximately 100 CFU/mL. Therefor an estimated colony count of 500 = 50,000 CFU/mL. Counts exceeding the above reporting levels are rounded up to the next level.

It is important to note that the enumeration of colonies is only an estimate, particularly when the number of colonies on the plate exceeds 100 (10,000 CFU/mL).

Urine cultures showing positive results are retained for one week, should identification and/or susceptibility testing be desired.
CPT coding 87205; 87088
Last Updated 11/14/2017 7:22:31 PM
Entry Number 101
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