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Urine Culture and Gram stain (to modify treatment prior to culture results)
|Available Stat?||No (gram stain may be requested stat)|
|Utilization Guidelines||Urine gram stains are indicated when the patient is septic and it is desirable to know the anatomic site of the primary infection. For simple UTI diagnosis urine culture w/o gram stain should be ordered.|
|Test group||Bacterial Culture|
|In House Availability||Set up daily, day and evening shifts|
|Collection Instructions||Submit voided urine in a cup with a screw-on lid. Use a Red top vacutainer to collect the specimen from a bladder catheter. Specify whether a Gram stain is desired. The physician can, by checking the appropriate box on the requisition, select a screening culture for significant numbers of a presumptive pathogen only and decline organism identification and susceptibility testing.|
|Container type||Urine cup|
|Amount to Collect||See preferred volume|
|Sample type||Random urine|
|Preferred volume||10 mL urine|
|UCSF Rejection Criteria||More than one midstream or indwelling cath urine in 48 hours|
|Normal range||See Additional Information|
|Stability||Refrigerated 24 hours|
|Reflex?||If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed.
Full ID and/or susceptibility testing may be omitted upon request when sample is submitted for testing.
|Additional information||Colony counts on urine cultures with growth are reported as follows:
Based on the use of a calibrated 10 µl loop used to inoculate plates, each resultant colony represents approximately 100 CFU/mL. Therefor an estimated colony count of 500 = 50,000 CFU/mL. Counts exceeding the above reporting levels are rounded up to the next level.
It is important to note that the enumeration of colonies is only an estimate, particularly when the number of colonies on the plate exceeds 100 (10,000 CFU/mL).
Urine cultures showing positive results are retained for one week, should identification and/or susceptibility testing be desired.
|Last Updated||6/19/2013 2:01:59 PM|