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Vitamin D, 25-Hydroxy

Item Value
Approval req'd? No
Available Stat? No
Test code 25HD
Performed by China Basin Chemistry
In House Availability Test performed Monday, Wednesday, Friday (day shift) only.
Method Diasorin Liason – Chemiluminescent Immunoassay
Container type Gold top
Amount to Collect 3 mL blood
Sample type Serum
Preferred volume 1.5 mL serum
Min. Volume 0.5 mL serum
Processing notes Freeze serum at -20C.
Units ng/mL
Normal range 30-100 ng/mL

Vit. D excess > 150 ng/mL
Vit. D sufficiency > 30 ng/mL
Vit. D insufficiency 10-30 ng/mL
Vit. D deficiency < 10 ng/mL
Synonyms 25-Hydroxycalciferol; 25-OH-D; Cholecalciferol Metabolite
Stability After separation from cells serum is stable at room temperature for 3 days, refrigerated 1 week and frozen at -20C 6 months.
Additional information To convert µg/L to nmol/L (SI units) multiply by 2.496.

Vit D excess > 150 ng/mL
Vit D sufficiency > 30 ng/mL
Vit D insufficiency 10 - 30 ng/mL
Vit D deficiency < 10 ng/mL


Reference ranges are based on:
1. Holick MF, Vitamin D status: measurement, interpretation, and clinical application, Annals of Epidemiology, 2009 Feb;19(2):73-8

2. Bischoff-Ferrari HA et al., Estimation of optimal serum concentrations of 25-hydroxyvitamin D for multiple health outcomes, Am J Clin Nutrition, 2006:84:18-28.
LOINC code 49054-0
Last Updated 10/15/2014 12:10:39 PM
Entry Number 1002
Lab Procedure Link Click here for Procedure
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