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Lab Manual for Moffitt-Long and Mount Zion

Lab Manual for SFGH

Internal Resources

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Urine Culture

Item Value
Approval req'd? No
Available Stat? No
Test code P059
Test group Bacterial Culture
Performed by Microbiology
In House Availability Set up daily, day and evening shifts
Method Aerobic culture
Collection Instructions Submit voided urine in a cup with a screw-on lid. Use a Red top vacutainer to collect the specimen from a bladder catheter. Specify whether a Gram stain is desired. The physician can, by checking the appropriate box on the requisition, select a screening culture for significant numbers of a presumptive pathogen only and decline organism identification and susceptibility testing.
Container type Urine cup (clean catch)
Red top (indwelling catheter)
Amount to Collect See preferred volume
Sample type Random urine
Preferred volume 10 mL urine
UCSF Rejection Criteria More than one midstream or indwelling cath urine in 48 hours
Normal range
Stability Refrigerated 24 hours
Reflex? If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed.

Full ID and/or susceptibility testing may be omitted upon request when sample is submitted for testing.
Additional information Colony counts on urine cultures with growth are reported as follows:

1,000 CFU/mL
10,000 CFU/mL
100,000 CFU/mL
≥1,000,000 CFU/mL

Based on the use of a calibrated 10 µl loop used to inoculate plates, each resultant colony represents approximately 100 CFU/mL. Therefor an estimated colony count of 500 = 50,000 CFU/mL. Counts exceeding the above reporting levels are rounded up to the next level.

It is important to note that the enumeration of colonies is only an estimate, particularly when the number of colonies on the plate exceeds 100 (10,000 CFU/mL).

Urine cultures showing positive results are retained for one week, should identification and/or susceptibility testing be desired.
CPT coding 87086 (culture & colony count), 87088 (Culture, Colony count, ID)
Medical Necessity? Yes. Click here for more information
LOINC code 630-4
Last Updated 6/19/2013 2:00:15 PM
Entry Number 100

If you have additional questions regarding this test, please call: 415-353-1667

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